Bacteria – 16S rDNA Test
Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.
Testing Indications
This test is intended to be used on clinical specimens from normally sterile sites in which bacterial infection is suspected however all routine culture methods (or specific molecular panels) have been negative. 16S rDNA testing on culture negative clinical samples requires approval from PHOL customer service centre before sample can to be submitted to lab. This is not a STAT test.
Specimen Requirements
Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
16S rDNA |
Tissue |
0.5 grams |
Sterile container Virus-Enteric kit Order # 390087 |
|
16S rDNA |
CSF |
250 ul |
Sterile container Virus-Enteric kit Order # 390087 |
|
16S rDNA |
Aspirate |
500 ul |
Sterile container Virus-Enteric kit Order # 390087 |
|
16S rDNA |
Body fluids |
500 ul |
Sterile container Virus-Enteric kit Order # 390087 |
|
16S rDNA |
Swabs |
500 ul |
Virus Respirarory Collection kit. Order number 390082 |
Submission and Collection Notes
This is not a STAT test. Call PHOL CSC- customer service centre at 1-877-604-4567/416-235-6556, Monday to Friday 8 am to 5 pm EST to obtain approval before submitting a sample for 16s rDNA testing. Provide information regarding sample type, clinical history, any other testing performed.
Specimens types not listed above (eg. fixed tissue, blood samples) must be discussed with the Microbiologist
Limitations
This is not a STAT test.
Failure to detect bacterial 16S rDNA does not rule-out the possibility of bacterial infection.
Samples with multiple species of bacteria present may be uninterpretable.
Identification of bacterial DNA from one species does not eliminate the possibility of DNA from other species being present in the sample (typically in lower amounts that may go undetected).
Test results must be correlated with patient’s clinical presentation, treatment history and other findings.
This is an laboratory-developed end-point PCR and Sanger sequencing based assay that has not been Health Canada cleared or approved.
Storage and Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Special Instructions
Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.
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