CPE – Carbapenemase Producing Enterobacteriaceae – Confirmation
Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.
Specimen Requirements
| Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
CPE – Carbapenemase producing Enterobacteriaceae |
Pure viable subculture of organism on appropriate medium (Columbia sheep blood agar plate) or a swab of a pure subculture of the organism in amies transport medium |
N/A |
N/A |
Submission and Collection Notes
Specimen Type: Pure viable subculture of 24 – 48 hours growth of organism on an appropriate medium or a swab for CPE confirmation test.
Limitations
Note: if a swab or broth is received the turnaround time will be delayed by at least 24 hours.
NB: Primary cultures are unacceptable as they should be processed in the originating lab. The submitter will be contacted by telephone. A report will be issued to indicate that the test has been rejected.
Storage and Transport
Label the specimen with at least 2 identifiers and place in a biohazard bag and seal.
Special Instructions
NB: Culture must be viable. Use a fresh subculture and ship in conditions to ensure viability on receipt.
Test Frequency and Turnaround Time (TAT)
CPE confirmation testing is performed daily.
Turnaround time is up to 3 days.
CPE confirmation is performed on Enterobacteriaceae using phenotypic inhibitor method and multiplex PCR assay.
Reporting
Results are reported to the ordering physician or health care provider as indicated on the requisition.
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