HCV Drug Resistance
Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.
Specimen Requirements
Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
HCV Drug Resistance1 |
Serum2 |
2.5 ml |
Blood - Serum Separator Vacutainer Tube (SST- yellow top) |
|
HCV Drug Resistance1 |
Plasma2 |
2.5 ml |
Plasma Vacutainer Tube (EDTA-lavender top) |
|
HCV Drug Resistance1 |
Not required for a previously tested sample3 |
Submission and Collection Notes
HCV drug resistance testing is performed when HCV RNA > 10,000 (1x10e+4) IU/ml
Remove serum or plasma from the clot/blood cells within 6 hours of collection and send to PHO Laboratory frozen.
Indicate on the requisition that the sample has been previously submitted and provide the PHO Laboratory number and fax the requisition to 416 235-6194
Limitations
Haemolysed, icteric, lipemic or microbially contaminated serum or plasma are not acceptable for testing.
Improperly collected, labelled, or processed specimens will be rejected.
If unable to process, separate and freeze serum or plasma within 6 hours of blood collection, a whole blood specimen (SST or EDTA) may be submitted to your local PHO Laboratory within 6 hours of collection who will then process and freeze the sample prior to testing.
Storage and Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Special Instructions
Centrifuge blood specimen. Serum or plasma must be removed from the clot within 6 hours of collection. Place specimen in a biohazard bag and seal. Specimens should be stored frozen at -20°C or colder and shipped to PHO laboratories using ice packs or dry ice in a proper shipping container.
Test Frequency and Turnaround Time (TAT)
HCV Drug Resistance testing is sent to British Columbia Centre for Excellence (BCCfE) weekly on Monday to Wednesday.
Turnaround time is up to 21 days from receipt by PHO Laboratory.
HCV Drug Resistance test is referred by PHO Laboratory to BCCfE.
HCV Drug Resistance test is performed on a “Research Use Only” basis. The methodology is based upon traditional Sanger-sequencing and includes identification of known mutations associated with drug resistance as well as a simple interpretation.
HCV RNA specimens must have > 10000 IU/mL HCV Viral Load for the HCV Drug Resistance test to be performed.
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