Herpes simplex – Conjunctival, Skin and genital

Testing Indications

The Herpes Simplex virus (HSV) polymerase chain reaction (PCR) test is a multiplex assay that detects HSV-1, HSV-2 and Varicella Zoster virus (VZV).

Storage and Transport

Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.

Special Instructions

Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.

Complete all fields of the General Test Requisition Form. 

Include, the patient’s full name, date of birth, Health Card Number (must match the specimen label), enter the name of the test requested under test description, source of specimen, date of onset, date of collection, physician name and address, and clinical diagnosis. 

Virus Culture Kit Order #390081; Order using Requisition for Containers and Supplies.

Test Frequency and Turnaround Time (TAT)

Herpes Simplex Virus polymerase chain reaction (HSV PCR) test will be run daily Monday to Friday.

Turn-around time is up to 4 days upon receipt at PHO’s laboratory.

HSV culture (ELVIS) has been temporarily suspended at PHO’s laboratory since June 2022 due to the emerging MPX (formerly monkeypox) in Ontario. HSV/VZV real-time PCR is performed for HSV detection and HSV typing instead.

Reporting

Results are reported to the ordering physician or health care provider as indicated on the requisition.

When herpes simplex virus is detected in cerebrospinal fluid (CSF) specimens, result is also reported to the Medical Officer of Health as per Health Protection and Promotion Act.