Nocardia – Aerobic Actinomycetes
Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.
Specimen Requirements
Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
Lab to complete |
Bronchial washings |
Sufficient sample must be received for both culture and microscopy to be performed. If insufficient sample is received, culture only will be done. |
Sterile container |
|
Lab to complete |
Sputum |
Sufficient sample must be received for both culture and microscopy to be performed. If insufficient sample is received, culture only will be done. |
Sterile container |
|
Lab to complete |
Bronchial alveolar lavage |
Sufficient sample must be received for both culture and microscopy to be performed. If insufficient sample is received, culture only will be done. |
Sterile container |
|
Lab to complete |
Abscesses |
Sufficient sample must be received for both culture and microscopy to be performed. If insufficient sample is received, culture only will be done. |
Sterile container |
|
Lab to complete |
Tissue |
Sufficient sample must be received for both culture and microscopy to be performed. If insufficient sample is received, culture only will be done. |
Sterile container |
Submission and Collection Notes
Note: Swabs are not accepted for culture.
Storage and Transport
Place specimen in a biohazard bag and seal.
Store bronchial washings, sputum, BAL and abscess at 4°C.
Store tissue specimens at room temperature.
Specimens should be shipped to the lab ASAP where possible.
Test Frequency and Turnaround Time (TAT)
The Nocardia specimen testing is done daily Monday to Friday.
Turnaround Time for microscopy: 1 day.
Turnaround Time for culture: within 4 weeks.
Turnaround Time for sensitivity: within 7 working days for positive culture submitted as pure growth.
Microscopy and culture for aerobic actinomycetes.
Microscopy: Gram stain and modified Acid Fast stains
Identification of aerobic actinomycetes from culture is performed using conventional methods including microscopic morphology, MALDI-TOF and molecular sequencing MLSA if needed.
Additional Information
Any aerobic Actinomycetes isolated from submitted specimens is forwarded to Toronto Public Health laboratory for identification. Please indicate on the requisition if the patient is immunocompromised or High Risk.
Susceptibility testing for Nocardia species (and aerobic actinomycetes) will be performed by broth microdilution assay on request.
Susceptibility results will be reported as MICs with or without interpretation as per CLSI guidelines. For guidance in interpretation of results, please contact the microbiologist.
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