Toxoplasma – Microscopy and PCR
Consistent with O. Reg. 671/92 of the French Language Services Act, laboratory testing information on this page is only available in English because it is scientific or technical in nature and is for use only by qualified health care providers and not by members of the public.
Specimen Requirements
Test Requested | Required Requisition(s) | Specimen Type | Minimum Volume | Collection Kit |
Toxoplasma Microscopy |
Biopsy material, tissue, lymph node aspirate |
2 ml |
Sterile container |
|
Toxoplasma PCR3 |
Amniotic fluid |
1 ml |
Sterile container |
|
Toxoplasma PCR3 |
EDTA Blood |
4 ml |
EDTA |
|
Toxoplasma PCR3 |
Bronchial Alveolar Lavage (BAL) |
10 ml |
Sterile container |
|
Toxoplasma PCR3 |
CSF |
1 ml |
Sterile container |
|
Toxoplasma PCR3 |
Pleural fluid |
1 ml |
Sterile container |
Submission and Collection Notes
Please note on the requisition (under other): If the patient has Eosinophilia, HIV, is immunocompromised, has a Critical illness or was admitted to ICU, or had a previous parasitic infection.
Indicate Country/Region and if the patient is a new immigrant or refugee or returned traveler under ‘Travel’ section of the requisition.
PHO laboratory refers samples for Toxoplasma PCR to the National Reference Centre for Parasitology (NRCP) - Room E03-5375, 1001 Décarie Blvd., Montreal, QC H4A 3J1.
Storage and Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Special Instructions
Place specimen container in a biohazard bag and seal. Ship to PHO laboratory ASAP.
Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs. Specimens must not be frozen.
Test Frequency and Turnaround Time (TAT)
Toxoplasma Microscopy testing is performed Monday to Friday. Turn-around time is up to 3 days from receipt by the PHO laboratory.
Samples for Toxoplasma PCR are shipped to NRCP Monday to Thursday.
Toxoplasma – Microscopy samples are examined by microscopy, using Giemsa-stained films.
Toxoplasma – PCR sample are tested by Polymerase chain reaction (PCR) to amplify DNA from T. gondii parasites present in body fluid.
Don’t have a MyPHO account? Register Now